Kaine, hoping to give the man who he thinks is the clone (Peter) the life he could never have, frames Ben for a number of murders he commits since both men have the same fingerprints. It is revealed that Spider-Gwen and Peter Parker of 616 saved Kaine, Ashley and Charlie, recruiting them for the final battle against the Inheritors on Earth-616. When Kaine sees Gwen Stacy being cloned, he breaks all three free before burning the Mark of Kaine into Jackal's face and breaking his neck. It is implied that Darkdevil does know the truth, however, once commenting on Kaine's "twisted notions of simple concepts like family".

In the comic's final story-arc, and a replica of May Parker appears on the scene, a comment is made about trying to find out which one is the real May Parker to which Kaine comments "Are you saying clones aren't real? As Verna learns she cannot absorb the life force of Jessica Drew due to her radioactive blood, Jessica plans to kill Verna. Like all Minimates figures, Kaine comes with a round stand with a peg designed for the normal Minimate foot. Despite the two engaging in a brutal confrontation — Kraven even briefly mistook Kaine for Spider-Man due to a vague recognition of Spider-Man's template in Kaine — both survive despite Kaine burying Kraven alive.From his former powers, Kaine appears to still have the "Mark of Kaine", using the ability to burn off his beard and most of his hair, and retains the two retractable stingers, one on the inside of each wrist, which retains its lethality and can tear through flesh and plastic. When the Jackal uses Reilly against Peter, the ensuing battle leaves Reilly and the Jackal for dead. Without his Scarlet Spider outfit, Kaine finds himself face-to-face with Ana Kravinoff, who tries to kill Kaine before informing him that her father Kraven the Hunter is coming for him and there is nothing he can do. This gives Kaine invisibility to both visual and audio means, along with immunity to sonic-based attacks. Kaine subsequently abandoned his plans to leave the city when the hospital where he left the victim was attacked by a fire-manipulating assassin who intended to kill the girl: Aracely.

Kaine convinces Jessica Drew of Earth-616 to join the team, and deceives her, telling her the mission is actually a suicide mission to destroy the stone containing Solus' essence that could potentially leave them trapped to die on the radioactive Earth-3145. Kaine made his way to Houston, where he found himself unable to stop from helping people in need as the costumed hero, Scarlet Spider.The set included Spider-Man, An unmasked Peter Parker Spider-Man, Scarlet Spider, Ben Reilly, Jackal, Sandman, Spidercide and Kaine.